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Md. Omar Faruk ¹, Dibyajyoti Saha ², Srikanta Chowdhury ¹, Swati Paul ², Md. Golam Kabir ¹

Affiliations
1 Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong-4331, BD
2 Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, BD

Abstract

The methanol crude extracts of the plant parts of Mimosa pudica (Mimosaceae) was screened was screened in vitro for antioxidant activity using the 1, 1-diphenyl-2-picrylhydrazyl-hydrate (DPPH) free radical scavenging assay. On the other hand, the methanol crude extract of the aerial part showed moderate antioxidant activity (IC50 20.512 μg/ml) compared to ascorbic acid (IC50 1.143 μg/ml). The overall experimental results suggest the biologically active constituents present in the methanolic extract of Mimosa pudica and justify its use in folkloric remedies.

Keywords

Mimosa pudica, Mimosaceae, DPPH, Antioxidant Activity.

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Srikanta Chowdhury ¹, Ramendu Parial ²

Affiliations
1 Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong -4331, IN
2 Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong -4331, BD

Abstract

Analyzing antibiotic susceptibility pattern of uropathogens help to overcome the therapeutic difficulties created by the rising antimicrobial resistant bacteria and guides in choosing appropriate antibiotics. The aim of the study was to estimate the prevalence of various bacterial isolates and to understand the susceptibility patterns of the uropathogens. Midstream urine samples were collected, cultured and subjected to microscopical and appropriate biochemical tests for proper identification. Antimicrobial susceptibility tests were carried out by disc diffusion technique using Muller Hinton Agar as culture media. The prevalence of urinary tract infection was 25.91%. It was more prevalent in woman than men (68% vs. 32%). The most common isolated uropathogens were Escherichia coli (82.6%) and Klebsiella pneumoniae (14.6%). Other bacterial species, named Morganella morganii, Enterobacter spp., Citrobacter freundii, Pseudomonas spp., Yersinia enterocolitica, were also found in patients with urinary tract infection, although they were least frequent. High level of sensitivity was found to imipenem, nitrofurantoin, meropenem, ceftazidime, netilmicin, gentamicin, chloramphenicol and amikacin in most of the isolates. Most of the bacterial isolates showed a higher percentage of resistance against amoxicillin, cefradine, ciprofloxacin, levofloxacin, ceftriaxone, co-trimoxazole and nalidixic acid. Multiple antibiotic resistance indexes suggested that almost all the test organisms exhibited multiple antibiotic resistances. The high multiple antibiotics resistance identified makes it necessary for antibiotic susceptibility testing to be conducted prior to antibiotic(s) prescription.

Keywords

Urinary Tract Infection, Uropathogens, Antibiotic, Susceptibility, Resistance.

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Srikanta Chowdhury ¹, T. A. Chowdhury ², Golam Kabir ², Dwaipayan Sikdar ², Aninda Kumar Nath ³, Zia Uddin ³, Dibyajyoti Saha ³

Affiliations
1 Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong-4331, BD
2 Department of Biochemistry and Molecular Biology, University of Chittagong, BD
3 Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, BD

Abstract

The aim of the present study was to obtain comparative data on chemical compositions and nutritive values of four different varieties of rice. The results indicated that moisture, ash, total soluble solid and crude fiber contents of the different varieties of rice were in the ranges of 9.98-10.90%, 9.45-11.12%, 10.06-11.25% and 9.35-10.35% respectively. The ranges in the values of total protein, water soluble protein, dry matter, polysaccharide, total sugar, reducing and non-reducing sugar compositions of rice were 12.45-14.06%, 3.20-4.71%, 89.10-90.02%, 10.95-12.25%, 4.35-4.96%, 1.15- 1.45%, and 3.10-3.58% respectively. Vitamin B1 and Vitamin B2 contents of rice were found to be ranged from 2.98-3.81 and 0.61-0.86 mg/100 gm of rice respectively. Twelve amino acids were detected in all of the four varieties of rice in the total form by paper chromatography. Rice contained about 14.95-16.16% oil. Triglyceride, diglyceride, monoglyceride, and nonglyceride were found to be varied from 91.76-93.08%, 1.51-2.30%, 1.45- 1.96% and 3.63-5.24% respectively. Rice oils contained mostly unsaturated fatty acid which was varied from 71.06-74.95%. Total lipids were fractionated into lipid classes by silicic acid column chromatography.

Keywords

Rice, Chemical Compositions, Nutritive Values, Chromatography, Fatty Acids.

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Srikanta Chowdhury ¹, T. A. Chowdhury ², Golam Kabir ², Dwaipayan Sikdar ², Aninda Kumar Nath ³, Zia Uddin ³, Dibyajyoti Saha ³

Affiliations
1 Department of Biochemistry and Molecular Biology, University of Chittagong, BD
2 Department of Biochemistry and Molecular Biology, University of Chittagong, BD
3 Department of Pharmacy, BGC Trust University, Bangladesh, Chittagong, BD

Abstract

The aim of the present study was to obtain comparative data on chemical compositions and nutritive values of four different varieties of rice. The results indicated that moisture, ash, total soluble solid and crude fiber contents of the different varieties of rice were in the ranges of 9.98-10.90%, 9.45-11.12%, 10.06-11.25% and 9.35-10.35% respectively. The ranges in the values of total protein, water soluble protein, dry matter, polysaccharide, total sugar, reducing and non-reducing sugar compositions of rice were 12.45-14.06%, 3.20-4.71%, 89.10-90.02%, 10.95-12.25%, 4.35-4.96%, 1.15- 1.45%, and 3.10-3.58% respectively. Vitamin B1 and Vitamin B2 contents of rice were found to be ranged from 2.98-3.81 and 0.61-0.86 mg/100 gm of rice respectively. Twelve amino acids were detected in all of the four varieties of rice in the total form by paper chromatography. Rice contained about 14.95-16.16% oil. Triglyceride, diglyceride, monoglyceride, and nonglyceride were found to be varied from 91.76-93.08%, 1.51-2.30%, 1.45- 1.96% and 3.63-5.24% respectively. Rice oils contained mostly unsaturated fatty acid which was varied from 71.06-74.95%. Total lipids were fractionated into lipid classes by silicic acid column chromatography.

Keywords

Rice, Chemical Compositions, Nutritive Values, Chromatography, Fatty Acids.

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Swati Paul ¹, Dibyajyoti Saha ¹, Srikanta Chowdhury ²

Affiliations
1 Department of Pharmacy, BGC Trust University Bangladesh, Chittagong, BD
2 Department of Biochemistry and Molecular Biology, University of Chittagong, BD

Abstract

The aerial part of Mimosa pudica (family: (Mimosaceae)) was studied to fix the parameters for pharmacognostical standards. The results of organoleptic study offer a scientific basis for the use of Mimosa pudica which possess characters like deep green colour, characteristic odour and slightly bitter taste. The fluorescence analysis under visible UV light by treatment with different chemical reagents showed different colour changes. These created an interest to test the possible phytochemical activity of the plant. In the screening process of Mimosa pudica indicate the presence of alkaloids, glycoloids, flavonoids, terpenoids, steroids, phlobatanins, tannins and saponins .The pharmacognostic study was performed by using standard procedure. Further, detailed studies are needed to know whether in-vivo administration of the extracts is beneficial for patients.

Keywords

Organoleptic, Phytochemical, Fluorescence, Mimosa pudica.

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